Techniques in Glycobiology: This course held at the Johns Hopkins University School of Medicine in 2019, 2021, and 2023 will cover a broad range of techniques used in the Glycosciences. The focus will be on the analysis of Glycolipids, Nucleotide Sugars, and the detection of the O-GlcNAc modification. A detailed schedule will be available soon.
Protocol to resolve High Molecular weight protein >1MDa
Proteoglycan and Mucin protein extraction
Tissue ganglioside by organic solvents
Protein Limiting Dilution Experiment
Chromatographic resolution by phase separation
High Throughput lectin binding array
Calculation for Multivalent binding
Immunostaining_Paraffin-embedded tissue section
O-GlcNAc Labeling: Release & Detection
Nucleotide Sugar Exraction & Analysis
OGA and OGT Assay